Trans women must be denied entry from female toilets, changing facilities and sports teams, new official guidance released today is expected to say. Equalities Minister Bridget Phillipson will state what businesses and public bodies must do under the law to protect single-sex spaces.

It follows last year’s Supreme Court ruling which found that the terms “woman” and “sex” under the Equality Act 2010 refer exclusively to biological sex. The government’s failure to publish it has meant that hospitals and leisure centres have allowed trans women into female spaces.

Helen Joyce, director of advocacy at Sex Matters, said: “There never was any reason for employers and service providers to wait for this guidance before implementing the law.

“The Supreme Court was completely clear that when providing single sex spaces and services, sex has to mean sex – male and female.

Speaking to The Telegraph, she added: “But once the Equality and Human Rights Commission’s (EHRC) new statutory guidance has been published, all delaying tactics must be over.

“All the organisations – including the NHS, local councils and government departments – which fell for the lie that equality law required them to allow people to self-identify their sex must now rewrite legally flawed policies.”

The guidance was written by the EHRC and submitted to Ms Phillipson in September but she asked for several revisions before agreeing to publish it, according to reports.

The EHRC has agreed to make a number of “tweaks”, including adding more examples on how organisations can ensure trans people have access to toilets and changing rooms while ensuring women’s facilities remain single-sex.

The guidance will apply to any organisation providing services to the public. It also covers private organisations or charities delivering public services.